Treatment FAQ

what type of graph would be most appropriate to graph mean number of colonies per treatment?

by Dr. Bernie Wisoky V Published 2 years ago Updated 2 years ago

What is the best statistics graph to represent the data?

The histogram is another best statistics graph to represent the data. We use it to express quantitative data. In this graph, the range of values is known as classes. If the classes contain the lower frequencies, they would have the shorter ones containing the higher frequency.

Which graph is used to represent continuous data?

Also, a histogram uses to represent the continuous data. Line graphs, bar graphs, and histograms represent the data on the x-axis and y-axis.

How to choose which type of graph to use?

How to Choose Which Type of Graph to Use? When to Use . . . . . . a Line graph. Line graphs are used to track changes over short and long periods of time. When smaller changes exist, line graphs are better to use than bar graphs.

How many types of graphs are there in qualitative research?

Let’s check all 7 graphs in detail. 1. Pareto Diagram or Bar Graph A Pareto diagram is also known as a bar chart. It is the best way to represent qualitative data. It was developed in the early 1900s by Vilfredo Pareto. He used this graph to conduct his study on wealth and poverty.

How do you choose which graph is most appropriate?

If you want to compare values, use a pie chart — for relative comparison — or bar charts — for precise comparison. If you want to compare volumes, use an area chart or a bubble chart. If you want to show trends and patterns in your data, use a line chart, bar chart, or scatter plot.

What type of graph would be best for showing means for several different treatment?

Line graphs are the best type of graph to use when you are displaying a change in something over a continuous range.

What type of graph would you use to show a population?

Population graphs are usually displayed as line graphs: graphs with an x-axis and a y-axis that have one continuous line running from left to right.

Which type of graph is better for showing distribution of data?

Scatter plotsScatter plots are best for showing distribution in large data sets.

What is a histogram vs bar graph?

Histograms are used to show distributions of variables while bar charts are used to compare variables. Histograms plot quantitative data with ranges of the data grouped into bins or intervals while bar charts plot categorical data.

When would you use a bar graph?

Bar graphs are used to compare things between different groups or to track changes over time. However, when trying to measure change over time, bar graphs are best when the changes are larger.

When would you use a scatter plot?

Use a scatter plot when you have two variables that pair well together. If you have two variables that pair well together, plotting them on a scatter diagram is a great way to view their relationship and see if it's a positive or negative correlation.

How do you know which graph to use in statistics?

time.” It is crucial to choose the correct graph type based on the kind of data to be presented. If the independent and dependent variables are numeric, use line diagrams or scattergrams; if only the dependent variable is numeric, use bar graphs; for proportions, use bar graphs or pie charts.

What is an area graph used for?

Area Graph Like Line Graphs, Area Graphs are used to display the development of quantitative values over an interval or time period. They are most commonly used to show trends, rather than convey specific values. Two popular variations of Area Graphs are: grouped and Stacked Area Graphs.

What type of graph is best to use to organize numerical data based on their digits to compare value?

Chapter 5 ~ Choosing Appropriate Data DisplaysABscatter plotused to see TRENDS in PAIRED NUMERICAL databar graphCOMPARE CATEGORICAL datacircle graphrepresent CATEGORICAL data as PARTS of a WHOLEstem-and-leaf plotsorganize NUMERICAL data based on their DIGITS5 more rows

Which type of display would be best to analyze these data?

Line graphs are used to display data or information that changes continuously over time. Line graphs allow us to see overall trends such as an increase or decrease in data over time. Bar graphs are used to compare facts.

What kind of data is a histogram used for?

A histogram is used to summarize discrete or continuous data. In other words, it provides a visual interpretation. of numerical data by showing the number of data points that fall within a specified range of values (called “bins”).

How many types of statistics graphs are there?

All these seven types of statistics graphs are the major ones. Apart from that, there are other types of statistics graphs, i.e., the statistics bar graphs, statistics, misleading graphs, and statistics line graphs.

Why are graphs useful?

Graphs are used to make the data more productive and unleash the hidden potential of data.

What is a circle graph?

A circle graph is also known as Pie charts. It is also one of the widely used statistics graphs in the world. Statisticians commonly used these graphs to represent the data graphically. As the name suggests, this graph looks like a circular pie with a few slices.

Why do graphs come into existence?

That is why the graphs come into existence to represent the aggregate statistic value in clean and well-managed order.

Why is statistics important?

We can’t utilize different data without the use of statistics. Therefore the significant role of statistics is to represent the data in a meaningful way. In this way, anyone can understand the data without in-depth ...

Why do you use different colors on a graph?

Use different colors to define different things on a graph. Sometimes, you need to represent the different data on a single graph. In that case, you can use varieties of colors to categorize or define the parameters. This makes your graph more understandable to the readers.

What is qualitative data?

Qualitative data means the data is not presented in numerical form. Besides, we put the different categories in each slice of the pie. The size of slices varies upon the data. Some slices might be more significant, and some might be smaller.

Why are graphs important in statistics?

That's where graphs can be invaluable, allowing statisticians to provide a visual interpretation of complex numerical stories. Seven types of graphs are commonly used in statistics. Good graphs convey information quickly and easily to the user. Graphs highlight the salient features of the data.

What type of data is used in graphs?

The type of data often determines what graph is appropriate to use. Qualitative data, quantitative data, and paired data each use different types of graphs.

What is a histogram graph?

A histogram in another kind of graph that uses bars in its display. This type of graph is used with quantitative data. Ranges of values, called classes, are listed at the bottom, and the classes with greater frequencies have taller bars.

What is a scatterplot in statistics?

A scatterplot usually looks like a line or curve moving up or down from left to right along the graph with points "scattered" along the line. The scatterplot helps you uncover more information about any data set, including:

How to do a scatterplot?

A scatterplot displays data that is paired by using a horizontal axis (the x-axis), and a vertical axis (the y-axis). The statistical tools of correlation and regression are then used to show trends on the scatterplot. A scatterplot usually looks like a line or curve moving up or down from left to right along the graph with points "scattered" along the line. The scatterplot helps you uncover more information about any data set, including: 1 The overall trend among variables (You can quickly see if the trend is upward or downward.) 2 Any outliers from the overall trend. 3 The shape of any trend. 4 The strength of any trend.

What is a dot plot?

A dot plot is a hybrid between a histogram and a stem and leaf plot. Each quantitative data value becomes a dot or point that is placed above the appropriate class values. Where histograms use rectangles—or bars—these graphs use dots, which are then joined together with a simple line, says statisticshowto.com. Dot plots provide a good way to compare how long it takes a group of six or seven individuals to make breakfast, for example, or to show the percentage of people in various countries who have access to electricity, according to MathIsFun .

Why are histograms different from bar graphs?

A histogram often looks similar to a bar graph, but they are different because of the level of measurement of the data. Bar graphs measure the frequency of categorical data. A categorical variable is one that has two or more categories, such as gender or hair color.

How many colonies are there in a microbial enumeration test?

The compendia have recently harmonized a microbial enumeration test (4), and in this test recommend that the technician �Select the plates corresponding to a given dilution and showing the highest number of colonies less than 250 for TAMC and 50 for TYMC.� In determination of the resistance of biological indicators, USP recommends a range of �20 to 300 colonies, but not less than 6� (5). However, the most complete description of the countable range is found in the informational chapter <1227> (6):

What is the fuss about in determining the number of colony forming units?

What is the fuss about in determining the number of colony forming units? After all, the CFU is only an estimate of the number of cells present. It is a skewed estimate at best as the only cells able to form colonies are those that can grow under the conditions of the test (incubation media, temperature, time, oxygen conditions, etc). Even among that group of microorganisms a colony does not represent a single cell, but rather cells that happened to be well separated on the plate and so can be distinguished after growth. A colony could arise from one cell, or several thousand. So why the fuss?

How many CFU per plate?

Their recommendation at the end of the study was for a countable range of 25-250 CFU/plate in triplicate. It is interesting to note that although the authors note that CFU follow a Poisson distribution, no mention is made of any data transformation used to approximate a normal distribution prior to the use of normal statistical analytical tools. Tomasiewicz et al provide excellent cautionary advice:

How many CFU per plate for Aspergillus niger?

The recommended range for Aspergillus niger is between 8 to 80 cfu per plate. The use of membrane filtration to recover challenge organisms, or the use of environmental isolates as challenge organisms in the antimicrobial effectiveness testing, requires validation of the countable range.�.

What is the ASTM recommendation for a 1:10 dilution?

ASTM recommendations focus on the LOD, and urge the user to report that answer out if no colonies are recovered (ie <10 CFU/mL for a 1:10 dilution) (7). If countable colonies are present, but below the countable range, count them anyway and report an estimated count.

How many colonies can grow on a plate?

The general ranges in common acceptance for countable numbers of colonies on a plate are 30 � 300 and 25 � 250. The origin of those ranges is worth examination. Breed and Dotterrer published a seminal paper on this topic in 1916 (2). They set out to determine the �limit in the number of colonies that may be allowed to grow on a plate without introducing serious errors�in connection with the proposed revisions of standard methods of milk analysis.� They note that �the kind of bacteria in the material under examination will have an influence on the size of the colonies, and consequently, on the number that can develop on a plate.� They also note that food supply can be an issue, that colonies close to each other on the plate may merge, and that neighbor colonies may inhibit growth or conversely stimulate growth. �Because of these and other difficulties certain plates in any series made from a given sample are more satisfactory for use in computing a total than are others. The matter of selecting plates to be used in computing a count becomes therefore a matter requiring considerable judgment.�

What is the central concern in this determination?

A central concern in this determination is the reporting of the Limit of Quantification (which is what we are really interested in reporting) against the Limit of Detection (1 CFU). This is an important distinction if we are being held to specifications in the lower range.

How many colonies grew after plating 10 -5?

Example 2: After plating 0.2 ml of a 10 -5 dilution, 183 colonies grew.

What is the most common type of statistical analysis done on laboratory data?

One of the most common types of statistical analysis done on laboratory data is determination of standard deviation. It is a useful measure of what is called the “scatter of observations,” giving an idea of how much importance to place upon variations in repeated observations or replicate experiments.

What is the most common dilution?

The most common dilutions are 1/10 and 1/100, but any dilution can be made. Because dilutions are large when counting bacteria, exponents are used. Answers should be written with two significant figures in proper scientific notation, i.e. 1.5 x 108 (Remember: 1 x 10 a = 10 a ). a.

How to find the standard deviation of a sample?

The standard deviation for each sampling point (column 4) is obtained by taking the positive square root of the deviation. This information can then be added to a graph, to illustrate the difference among the five batches of culture.

How to determine generation time?

You can use the graph to determine the generation time. Choose two points on the graph between which the population doubled and determine the time it took for this to happen. For example at 0 time the cell number was 4 X 10 7 CFU/ml and at 90 minutes it was 8 X 10 7 CFU/ml, so the generation time was 90 minutes.

How to find sample mean for E. coli?

The sample mean for these data is achieved by adding all the average CFU/ml (column 2) and dividing by the number E. coli cultures grown (column 1):

Why are TNTC counts considered too many?

Counts above 250 are considered Too Numerous To Count (TNTC) because it is impossible to tell whether colonies are separated. Plates with less than 25 colonies do not have a statistically significant number of colonies. When the approximate number of bacteria is unknown, plate a wide range of dilutions. In this way you will have at least 1-2 plates ...

Who Cares?

Countable Range on A Plate

  • Literature
    The general ranges in common acceptance for countable numbers of colonies on a plate are 30 � 300 and 25 � 250. The origin of those ranges is worth examination. Breed and Dotterrer published a seminal paper on this topic in 1916 (2). They set out to determine the �limit in the n…
  • Upper Limit
    The upper limit of plate counts is dependent on a number of factors, as described previously. The major issues include the colony size and behavior (swarming?), and the surface area of the plate. The size particularly comes into play with plating a membrane for determination of CFU as the s…
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Rounding and Averaging

  • To discuss this we need to determine what the significant figures might be in the measure. For raw colony counts, common practice determines that the CFU observed determine the significant figure, and that the average is one decimal to the right of that number (sticklers for accuracy will report the geometric mean rather than the arithmetic mean given the Poisson distribution follow…
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Impact on Specifications and Environmental Monitoring Control Levels

  • We are back to the question of WHO CARES? If you are faced with a finished product bioburden of NMT (Not More Than) 100 CFU/gram, and your method suitability study requires a 1:100 dilution of the product to overcome any antimicrobial effects, then how are you to test it? Common practice is to perform the 1:100 dilution, perform a pour plate of 1 mL in duplicate and if 2 coloni…
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Conclusions

  • In conclusion, all methods have limitations. One of the major limitations to the plate count method is the relatively narrow countable range (generally considered to be 25-250 CFU bacteria on a standard petri dish). The currently prevailing confusion between the Limit of Detection (1 CFU) and Limit of Quantification (25 CFU) for the plate count method creates a larger degree of variab…
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References

  1. Pharm Eur. 2006. 5.1.6 Alternative Methods for Control of Microbiological Quality. Pharm Eur vol.5.5 pp 4131-4142.
  2. Breed, R and WD Dotterrer. 1916. The Number of Colonies Allowable On Satisfactory Agar Plates. J Bacteriol. 1:321-331.
  3. Tomasiewicz, D.M.et al. 1980. The Most Suitable Number of Colonies On Plates for Counting…
  1. Pharm Eur. 2006. 5.1.6 Alternative Methods for Control of Microbiological Quality. Pharm Eur vol.5.5 pp 4131-4142.
  2. Breed, R and WD Dotterrer. 1916. The Number of Colonies Allowable On Satisfactory Agar Plates. J Bacteriol. 1:321-331.
  3. Tomasiewicz, D.M.et al. 1980. The Most Suitable Number of Colonies On Plates for Counting. J Food Prot. 43(4):282-286.
  4. USP. 2006. <61> Microbial Examination of Nonsterile Products: Microbial Enumeration Tests. USP 29 Suppl 2. August 1, 2006 . United States Pharmacopeial Convention. pp. 3757-3759.

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