Full Answer
Do I need to acid wash my coverslips?
Soak coverslips in 6M to 10M nitric acid for 20 min. Using tweezers, remove the coverslips from the acid bath and wash 8 times with double-distilled water. Dry coverslips in a tissue culture hood and steralize as per steps 8-10 in the hydrochloric acid method, above. Neutralise and dispose of the acid using appropriate methods.
What is nitric acid and how does it work?
Nitric acid (HNO₃) is a colorless liquid with yellow or red fumes with an acrid odor. Exposure to nitric acid can cause irritation to the eyes, skin, and mucous membrane; it can also cause delayed pulmonary edema, pneumonitis, bronchitis, and dental erosion. Nitric acid is highly corrosive. Workers may be harmed from exposure to nitric acid.
How do you remove NOx from nitric acid?
Feb 24, 2015 · Some say 1) to prevent the sample from drying out 2) to prevent oxidation 3) to prevent the mounting medium from leeching onto the objective or 4) to keep the coverslip in place. It’s a mixture of all those reasons, but the reason varies depending on the type of mounting medium and the time after mounting.
What is homeopathic nitric acid used for?
In most cases the nitric acid needs to be free of NO x so that a bleaching respectively stripping process with air is the subsequent process step to get clear concentrated nitric acid. The generated NOx-gases are convertered into nitric acid and recycled to improve the over all process efficiency and to avoid NOx emissions. Nitric acid used for nitration processes is commonly …
How are coverslips treated in cell culture?
Place sterilized coverslips into the wells of a 24-well plate. Add 400 µL of the gelatin-coating solution, and incubate the coverslips for 10 minutes at room temperature. Remove the gelatin-coating solution, and air dry the coverslips for 15 minutes. The dried coverslips can now be stored at room temperature until use.
How do you clean microscope slides and coverslips?
When slides get soiled, you can clean them with soapy water or isopropyl alcohol. Do not immerse slides in water or soak them in it. This loosens the cover glass adhesive, causing the cover glass to come off and possibly ruin the slide.
How do you wash coverslips?
Wash the coverslips extensively in distilled water (two times), then double distilled water (two times). Be sure to wash out the acid between sticked coverslips. Rinse coverslips in 100% ethanol and dry them between sheets of whatman filter paper. Keep coverslip in a clean container.
Can you reuse glass coverslips?
If you're using cover slips, you should also wash those between every use. Johnny L. The common type of microscope slides are the simple glass ones used for compound light microscopes, and yes, they can be used repeatedly. Just make sure you wash and dry the slide very well between each use.Dec 8, 2014
What do you clean microscope lenses with?
Dip a lens wipe or cotton swab into distilled water and shake off any excess liquid. Then, wipe the lens using the spiral motion. This should remove all water-soluble dirt.
How do you remove oil from slide immersion?
0:182:10Hematology: Cleaning the Immersion Oil off a Slide - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd you're just going to gently Pat it up and down just up and down all right so that were bringingMoreAnd you're just going to gently Pat it up and down just up and down all right so that were bringing up that oil into the chem way notice how its oily now. And you know it looks bubbly on the slide.
Do you need to acid wash coverslips?
Therefore, coverslips should routinely be washed with acid or base solutions to rid them of this film. Commercial precleaned slides are also likely to be dirty and must be washed prior to use.
How do you autoclave coverslips?
Simply place the coverslips in a glass petri dish and send through the dry cycle of an autoclave. This is typically a 20 minute step. Some protocols advocate a 70% ethanol wash beforehand, but autoclaving alone will ensure that the glass is tissue culture sterile.Jun 12, 2015
How long can poly-L-lysine coating slides be stored?
Store diluted Poly-L-Lysine solution in refrigerator (2-8°C). The diluted solution is stable for at least three months. Filter diluted solution after use.
Why do you close the iris before you look through the Oculars?
In practice, you can experiment with this while viewing a specimen and adjust it without removing the ocular. Closing the aperture iris also increases depth of focus up to a point. Thus, more areas of a three dimensional specimen will be in focus If it is closed to much, a flat indistinct image results.
How do you disinfect a microscope slide?
Samples are placed on thin pieces of glass called microscope slides and covered with thin slivers of glass called coverslips.Glass slides can be cleaned with 70% ethanol and dried with a Kimwipe (lint free tissue) or lens tissue.Coverslips are fragile - handle with care.Coverslips should be clean and fingerprint free.More items...
How long do you expect a wet mount microscope slide to last before it dries out?
A well prepared slide will last for 15-30 minutes before it dries up. To extend the life of a wet mount slide, scrape petroleum jelly onto each of the four edges of the cover slip (Figure 2).
What is nitric acid?
Overview. CAS No. 7697-37-2. Nitric acid (HNO₃) is a colorless liquid with yellow or red fumes with an acrid odor. Exposure to nitric acid can cause irritation to the eyes, skin, and mucous membrane; it can also cause delayed pulmonary edema, pneumonitis, bronchitis, and dental erosion. Nitric acid is highly corrosive.
What are some examples of workers exposed to nitric acid?
Some examples of workers at risk of being exposed to nitric acid include the following: Factory workers in plants that produce nitric acid. Factory workers in plants that manufacture explosives. Workers in facilities that use nitric acid.
What is the DHHS standard for nitric acid exposure?
Criteria for a Recommended Standard: Occupational Exposure to Nitric Acid —DHHS (NIOSH) No. 76-141. Presents a standard to prevent the adverse effects of exposure to nitric acid over a working lifetime.
What is nioshtic 2?
NIOSHTIC-2 search results for nitric acid —NIOSHTIC-2 is a searchable database of worker safety and health publications, documents, grant reports, and journal articles supported in whole or in part by NIOSH.
Is nitric acid corrosive?
Nitric acid is highly corrosive. Workers may be harmed from exposure to nitric acid. The level of exposure depends upon the dose, duration, and work being done. Nitric acid is used in many industries. It’s used in the manufacture of fertilizers, dyes, and explosives. Nitric acid is also used in the polymer industry.
What is the best way to clean coverslips?
Acid Washing. Acid washing is often recommended by the manufacturers of coverslips, such as Corning, because it is not only reported to enhance the cleanliness of the coverslip but the acid also etches the glass making it a better substrate for cellular attachment.
Why do people paint around the edges of their coverslips?
Why do people bust out the nail polish and paint it around the edges of their coverslips? Some say 1) to prevent the sample from drying out 2) to prevent oxidation 3) to prevent the mounting medium from leeching onto the objective or 4) to keep the coverslip in place. It’s a mixture of all those reasons, but the reason varies depending on the type of mounting medium and the time after mounting. If you are using a mounting medium that does not harden, it might not make any difference when you seal it. But if you are using one that hardens, such as Prolong gold, and you seal it before it hardens, it may never cure properly. So make yourself familiar with the recommendations for your mounting medium, and also to check if there are any reports of the nail polish quenching your fluorophore of choice
What is poly-L-lysine used for?
This is a tried and true method. Coating with poly-L-lysine provides a positive charge for cell attachment and is used for a wide variety of cell types. If you are working with a cell type that does not attach to glass easily, like HEK293 cells, coating your coverslips with poly-L-lysine can make the difference between having one cell left after treatment, or a million. Some prefer to use poly-D-lysine since it is resistant to breakdown by cell-released proteases. Regardless, it IS important to spend the time rinsing off excess poly-lysine to prevent its accumulation into the media which induces cellular toxicity.
How long does it take to make coverslips?
There are about as many protocols to prepare coverslips as there are ways to make tuna casserole. You can spend from 5 seconds to 2 days, depending on what your lab prefers. But in the end, what’s really needed?
Is autoclaving tissue culture sterile?
The upside of autoclaving is that you are sure your coverslips are tissue culture sterile, which is necessary if you plan on growing cell s on these . The downside is that autoclaving takes time. Also, don’t autoclave fancy commercially-purchased precoated coverslips.
How long does it take to incubate under UV light?
Incubations under the UV light vary from 15 minutes, up to 1 or 4 hours, or even overnight. The only negative to this method is that your lab mates are going flock to that blue light like a moth and will be annoyed that the hood isn’t being used for tissue culture work.
Can you use PBS to rinse coverslips?
Some people recommend rinsing your coverslips in PBS instead of water. And while the coverslip does appear to coat better when there is salt in the water, I do not use PBS. Instead I use pure water because I do not want the possibility of salts drying onto the coverslips, since salt could carry over and cause problems in future steps.
What is nitric acid?
Introduction. Nitric acid is one of the most common mineral acids in the chemical Industry. It is produced by a high temperature oxidation of ammonia with oxygen catalyzed by a platinum/rhodium contact providing with water nitric acid with a strength of up to almost 68wt% can be generated. The majority of this nitric acid is used for ...
Why is nitric acid not reacting?
The nitric acid thereof is not reacting quantatively during the nitration process so that the recovery of the acids is important for the cost efficiency of the overall nitration process and for the environment reducing nitrates in waste waters.
What is the name of the mixture that is used to make NOx gas?
Nitric acid used for nitration processes is commonly mixed with sulfuric acid to become a mixture called nitration acid .
What is nitric acid used for?
With this process the latent medicinal properties of Nitric Acid are extracted. It has been used since ages to treat warts and skin ulcers. In homeopathy, it very effectively manages complaint of anal fissures, piles, warts and mouth ulcers.
How often can you take nitric acid?
Nitric Acid can be used in 30 C and 200C potency. One can take its 30 C potency once a day, while its 200 C potency can be taken weekly.
What does it mean when your tonsils are red?
The throat and tonsils are red, swollen and highly inflamed. Small ulcers also appear on tonsils and in the throat. The ulcers are usually deep with bluish margins and red centre. It is attended with offensive smell from mouth. 2.
What is the name of the disease that causes yellow discharge?
This medicine is of great service in treating gonorrhoea in males. Gonorrhoea refers to a sexually transmitted disease caused by a bacteria called Neisseria gonorrhoea. It is used when there is a yellowish or greenish discharge from urethra. Along with this ,there is severe pain in urethra on urination.
What is a pile in the rectum?
Next, it is very helpful to treat piles. Piles are the swollen and inflamed veins in the rectum and anus. Persons who require it have pain in the piles. The pain experienced by them is sharp, stitching, pricking and splinter like pain. The piles are also painful to touch.
Where are ulcers on the tongue?
For using it here the ulcers are present within cheeks, on the tongue and on the lips. Stinging, pricking and burning pains are felt in the ulcers. Along with this there is increased salivation. Foul smell from mouth is also present with above symptoms.
What is the role of homeopathic medicine?
Role as a Homeopathic Medicine. 1. Throat Complaints. If we talk about throat complaints it is valuable to treat sore throat and tonsillitis. It is given when person have pain in the throat and tonsils. The pain is stitching, splinter like and burning type. The pain may extend to ear.
What are the symptoms of nitric acid?
Symptoms. Expand Section. Symptoms from swallowing nitric acid may include: Abdominal pain - severe. Burns to skin or mouth. Drooling. Fever.
How to contact poison control center?
Your local poison control center can be reached directly by calling the national toll-free Poison Help hotline (1-800-222-1222) from anywhere in the United States. This national hotline will let you talk to experts in poisoning. They will give you further instructions. This is a free and confidential service.
What is the number to call for poisoning?
If you or someone you are with has an exposure, call the local emergency number (such as 911), or the local poison center can be reached directly by calling the national toll-free Poison Help hotline (1-800-222-1222) from anywhere in the United States.
How much milk should I give to someone who swallowed a poison?
Give 4 to 6 ounces (120 to 180 milliliters) of milk of magnesia, if possible.
What is an ECG?
ECG (electrocardiogram, or heart tracing) Fluids through a vein (intravenous or IV) Medicines to treat symptoms. Tube through the nose into the stomach to suction (aspirate) any remaining acid if the person is seen shortly after the exposure and a large amount was swallowed.
How often should I wash my skin after a burn?
Transfer to a hospital that specializes in burn care. Washing of the skin (irrigation), possibly every few hours for several days. Hospital admission may be necessary to continue treatment. Surgery may be required if the esophagus, stomach, or intestines have developed holes (perforation) from exposure to the acid.
What tests are done to see if you have a burn in your throat?
The person may receive: Airway support, including oxygen, breathing tube through the mouth (intubation), and breathing machine (ventilator) Blood and urine tests. Camera down the throat to see burns in the airway (bronchoscopy) Camera down the throat (endoscopy) to see burns in the food pipe (esophagus) and stomach.
What is nitric acid used for?
Nitric acid is the building block chemical for the production of many other chemical compounds. It is used in manufacturing several types of polymers like polyamides and polyurethane. Nitric acid is also commonly used as rocket propellants in the aerospace industry. It is also used for manufacturing nitrogen-based compounds like nylon as well as ...
How is nitric acid made?
Nitric acid also called spirit of niter or aqua fortis comes with molecular formula of HNO 3 which consists of three oxygen atoms, one nitrogen atom and one hydrogen atom. Nitric acid is usually obtained by the oxidation of ammonia. It is mainly done using the Ostwald process. Nitric acid is also formed by treating sodium nitrate ...
Is nitric acid a strong oxidizing agent?
It is both extremely corrosive and toxic. Nitric acid is soluble in water and it is also a strong oxidizing agent. It reacts with metals, oxides, and hydroxides, forming nitrate salts.
What is the yellowing of the skin at the site of an engraving?
The fluid in question is nitric acid, also known as engraver's acid. Engravers often use this acid at a concentration of 60 to 70 percent. The diagnostic key here is the yellowing of the skin at the injury site. This yellowing is characteristic for chemical injury secondary to nitric acid exposure.
Why is my skin yellowing?
This yellowing is characteristic for chemical injury secondary to nitric acid exposure. Nitric acid is corrosive, and can cause severe burns. Its vapor is corrosive to the respiratory tract, and may cause pulmonary edema that could prove fatal. On contact with skin, liquid splashes with nitric acid may produce severe burns.
Can nitric acid cause a sore throat?
Following ingestion, nitric acid may cause immediate corrosion of and damage to the gastrointestinal tract. In addition, exposure to nitric acid fumes may be corrosive to the respiratory tract, and exposure may cause intractable coughing, sore throat, and difficulty breathing.
What supplements increase nitric oxide?
These supplements often contain several ingredients that are said to increase nitric oxide, such as nitrate or the amino acids L-arginine and L-citrulline.
What is nitric oxide?
The Bottom Line. Nitric oxide is a molecule that plays many important roles in human health. Many supplements are claimed to increase nitric oxide in the body and provide impressive benefits for health and performance. They usually contain ingredients like nitrate or the amino acids L-citrulline and L-arginine.
How long does it take for citrulline to reduce muscle soreness?
Those given citrulline malate reported 40% less muscle soreness at 24 and 48 hours after the exercise, compared to those who took the placebo. Citrulline malate increases nitric oxide production, which increases blood flow to active muscles.
What is the role of nitric oxide in blood flow?
It’s a vasodilator, meaning it relaxes the inner muscles of your blood vessels, causing the vessels to widen. In this way, nitric oxide increases blood flow ...
What is the name of the amino acid that helps with erectile dysfunction?
Erectile dysfunction (ED) is the inability to achieve or maintain an erection firm enough for sex ( 1. Trusted Source. ). L-citrulline is an amino acid that may help treat erectile dysfunction by increasing the production of nitric oxide ( 2. Trusted Source.
How does nitric oxide help with exercise?
Nitric oxide is involved in many cell processes, including the widening of the blood vessels, or vasodilation. Wider blood vessels help increase the delivery of nutrients and oxygen to working muscles during exercise, thus enhancing exercise performance.
What is the compound that causes blood vessels to relax?
Nitrate. Nitrate is a compound found in beetroot and dark leafy greens like spinach and arugula. When you consume nitrate , your body converts it to nitric oxide, which in turn causes blood vessels to relax and dilate, lowering blood pressure.
