Another group of researchers offered mice — nocturnal animals — food and water for 22 hours per day, but nothing but a bottle of alcohol for two hours during the night. Mice that like to drink would sip from the alcohol bottle, gradually increasing their consumption over the course of several days.
Full Answer
Can mice drink water with antibiotics in it?
Antibiotics especially Metronidazole and Ampicillin give unpleasant taste to drinking water. So, it is but natural for mice not to consume such water. Administration of antibiotics by gavage should be considered.
What is the best way to treat water poisoning in mice?
The best method is gavage feeding administration of antibiotics in mice. It is highly effective. Antibiotics especially Metronidazole and Ampicillin give unpleasant taste to drinking water. So, it is but natural for mice not to consume such water.
How much antibiotics do mice consume?
In addition, mice consumed similar volumes of antibiotic-treated and untreated water. The highest plasma antibiotic concentrations measured were: enrofloxacin, 140.1 ± 10.4 ng/mL; doxycycline, 56.6 ± 12.5 ng/mL; amoxicillin, 299.2 ± 64.1 ng/mL; and trimethoprim–sulfamethoxazole, 5.9 ± 1.2 ng/mL.
How do you measure daily water consumption in mice?
The mice were weighed at the start of the study, and daily water consumption was measured by weighing the water bottles for each pair of mice for 7 d. On day 7, the mice were weighed again, and the antibiotic was added to fresh tap water.
How do you administer drugs to mice?
Among several possibilities for the administration of substances to mice, the most common routes are subcutaneous, intraperitoneal or intravenous injection. The intramuscular administration is not recommended, as the muscle of the mouse is too small.
Why are mice used in drug research?
In many ways, mice are ideal for scientific experiments. They are genetically similar to humans, produce many offspring, and mature quickly so that experiments can be done in a short time period. They have helped to develop treatments for numerous diseases, from diabetes to cancer.
How do you handle mice in research?
Picking up mice at the base of the tail is standard practice in laboratory research, but whether this is the best method is unclear. Researchers now suggest that cupping a mouse in the hand or carrying it in a small tunnel reduces stress and encourages cooperation.
What experiments are done on mice?
More than 110 million mice and rats are killed in U.S. laboratories every year. They are abused in everything from toxicology tests (in which they are slowly poisoned to death) to painful burn experiments to psychological experiments that induce terror, anxiety, depression, and helplessness.
Why do researchers use female mice?
Why are female mice under-appreciated? The under representation of female mice in biomedical research is based on the assumption that females are intrinsically more variable than males.
Is it ethical to test on mice?
Against animal experiments: Experimenting on animals is always unacceptable because: it causes suffering to animals. the benefits to human beings are not proven. any benefits to human beings that animal testing does provide could be produced in other ways.
Does it hurt a mouse to hold it by its tail?
Jane Hurst of the University of Liverpool and a lead author of the study, tells Inverse. “The tail technique doesn't hurt mice, but it does make them anxious. To a mouse, being lifted by the tail and carried backwards feels very much like being caught by a predator.”
Is it OK to pick up mice by the tail?
Mice tails are quite fragile, so your grip should be just firm enough to keep your pet from scampering off. Do not, under any circumstance, pick your mouse up by its tail. Doing so will cause your pet lots of discomfort and, in some cases, can lead to major injuries like degloving.
How do I immobilize my mouse?
Mice can be safely restrained by grasping firmly at the base of the tail. This form of restraint is suitable for moving the mouse over a short distance, animal identification and weighing. For greater control such as during examination, or injections, place the mouse on a surface it can grasp.
Do mice feel pain when poisoned?
Poisoning causes a painful death to mice and any cats, dogs, or other animals who either ingest the poison by mistake or eat the bodies of rodents who have been poisoned. 3. Snap traps can prolong suffering. All mammals, regardless of species, have the same capacity to experience pain and terror.
What kind of research are mice used in?
They are the most commonly used mammalian research model and are used for research in genetics, physiology, psychology, medicine and other scientific disciplines. Mice belong to the Euarchontoglires clade, which includes humans.
Can mice feel pain?
Healthy mice living in the same room with mice experiencing pain are up to 68% more sensitive to pain themselves, regardless of their stress levels, according to the new study, which found that mice could scent when their fellows were suffering.
What happens if a rodent drinks less than 90% of its water?
If rodents drink less than 90% of their previous intake, become dehydrated, or lose weight, the PI must adjust the drinking water with additives so as to make it palatable for the animal. If the problem persists, the PI must consult with a BU ASC veterinarian.
Why is sucrose added to drinking water?
A compound such as sucrose may be added to the drinking water in order to make the experimental compound or medication more palatable (see Antibiotics Added to the Drinking Water in the Procedures section, below).
How long does sulfamethoxazole stay in water?
Both Sulfamethoxazole-Trimethoprim and Enrofloxacin were assessed for stability in drinking water and found to be stable up to 7 days. Plasma concentrations of the drugs were notably lower when administered parenterally. 3
What is the critical factor to be determined when any compound is added to the drinking water?
A critical factor to be determined when any compound is added to the drinking water is the animals’ fluid intake. It must be assured that fluid intake will not be diminished and the animals will not become dehydrated because of any new taste, smell, texture, or other factor altering the drinking water.
Most recent answer
The best method is gavage feeding administration of antibiotics in mice. It is highly effective.
All Answers (4)
Antibiotics especially Metronidazole and Ampicillin give unpleasant taste to drinking water. So, it is but natural for mice not to consume such water. Administration of antibiotics by gavage should be considered.
Similar questions and discussions
Hey everybody, I need help with a project I am working on with biofilm and Ti02 nanoparticle antimicrobial activity.
Where to inject a mouse?
The best place to inject is into the loose skin on the back of the neck. A mouse may easily be injected by one person, whereas a rat may require restraint by one person and injection by the other. Anesthesia is not required. Contact BU ASC at [email protected] for training in this procedure.
What is the responsibility of the investigator when adding additives to food?
Additives to the food: When additives are placed in the food it is the responsibility of the investigator to monitor the animal (s) and assure that adequate food intake occurs. Intragastric via gavage: The investigator must be trained and experienced in this procedure prior to undertaking it.
Can you inject ketamine into a mouse?
Any irritating compound, such as ketamine or pentobarbital, is less irritating if administered IP. A mouse may easily be injected by one person, whereas a rat may require restraint by one person and injection by the other. Anesthesia is not required. Contact BU ASC at [email protected] for training in this procedure.