How many samples are there in an experiment with 3 replicates?
Normally we design experiment with 3 replicates, each replicate has like 10 samples/treatment (so total number of samples n = 30/treatment). Then we average the results of these 10 samples to get 1 number/replicate and use these 3 numbers/treatment to performing statistical analysis.
How many replicates should be used to determine the actual value?
(In out of specification testing, where retesting is required to determine the actual value of a suspect sample with a high level of confidence, the number of replicates is usually somewhere between 5 and 8 for the same reason.)
How many runs does it take to replicate an entire design?
You can replicate combinations of factor levels, groups of factor level combinations, or entire designs. For example, if you have three factors with two levels each and you test all combinations of factor levels (full factorial design), one replicate of the entire design would have 8 runs (2 3 ).
Do I need a replication sample for my study?
Please also explain how you arrived at the answer. No. Of repli Join ResearchGate to ask questions, get input, and advance your work. For small studies, an independent replication sample is necessary.
What are the two types of replicates?
Why is replication used in sample preparation?
Do you use standard injections during chromatography?
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Replicates and repeats—what is the difference and is it significant?
Let us suppose we are testing the hypothesis that the protein Biddelonin (BDL), encoded by the Bdl gene, is required for bone marrow colonies to grow in response to the cytokine HH-CSF. Luckily, we have wild-type (WT) and homozygous Bdl gene-deleted mice at our disposal, and a vial of recombinant HH-CSF. We prepare suspensions of bone marrow cells from a single WT and a single Bdl −/− ...
IUPAC - replicate (duplicate) sample (R05299)
Multiple (or two) samples taken under comparable conditions. This selection may be accomplished by taking units adjacent in time or space. Although the replicate samples are expected to be identical, often the only thing replicated is the act of taking the physical sample.
Replicates and repeats in designed experiments - Minitab
Repeat and replicate measurements are both multiple response measurements taken at the same combination of factor settings; but repeat measurements are taken during the same experimental run or consecutive runs, while replicate measurements are taken during identical but different experimental runs, which are often randomized.
What are the two types of replicates?
Most commonly it comes down to two types, either preparation replicates or measurement replicates. In the case of preparation, these are samples or standards that are prepared from the beginning to end of the procedure in ...
Why is replication used in sample preparation?
When replicates are used in sample preparation it is more likely that the reason is related to variability of the method caused by the inherent errors in the sample preparation steps. When the sample is very simple, such as a pure substance then the replication may be used a weighing check as discussed for standards previously.
Do you use standard injections during chromatography?
This may vary quite significantly with some analysts preferring to use all injections of standard during the analysis (in the case of a chromatographic run where there are regular injections of standard in between injections of samples) and others using injections of standard prior to or bracketing the samples being analysed.
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For small studies, an independent replication sample is necessary. If the findings hold in independent replication, then the confidence in the results is considerably higher and may extend the literature base.
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You need to use the tool named "Power and size of the sample" in an statistical software as Minitab.
Similar questions and discussions
What is: “Experimental Unit”, “Replicate”, “Total sample size”, “treatment size”?
What is replicate in a study?
Replicates are multiple experimental runs with the same factor settings (levels). Replicates are subject to the same sources of variability, independently of each other. You can replicate combinations of factor levels, groups of factor level combinations, or entire designs. For example, if you have three factors with two levels each ...
Why is variability greater for replicates than for repeats?
The variability between measurements taken at the same factor settings tends to be greater for replicates than for repeats because the machines are reset before each run, adding more variability to the process.
What is the difference between repeat and replicate?
Repeat and replicate measurements are both multiple response measurements taken at the same combination of factor settings; but repeat measurements are taken during the same experimental run or consecutive runs, while replicate measurements are taken during identical but different experimental runs, which are often randomized.
How many measurements are taken in each experiment?
In each experiment, five measurements are taken at each combination of factor settings. In the first experiment, the five measurements are taken during the same run; in the second experiment, the five measurements are taken in different runs. The variability between measurements taken at the same factor settings tends to be greater ...
What are the two types of replicates?
Most commonly it comes down to two types, either preparation replicates or measurement replicates. In the case of preparation, these are samples or standards that are prepared from the beginning to end of the procedure in ...
Why is replication used in sample preparation?
When replicates are used in sample preparation it is more likely that the reason is related to variability of the method caused by the inherent errors in the sample preparation steps. When the sample is very simple, such as a pure substance then the replication may be used a weighing check as discussed for standards previously.
Do you use standard injections during chromatography?
This may vary quite significantly with some analysts preferring to use all injections of standard during the analysis (in the case of a chromatographic run where there are regular injections of standard in between injections of samples) and others using injections of standard prior to or bracketing the samples being analysed.