
- Aerobic Bacteria. Aerobic bacteria are mostly used in new treatment plants in what is known as an aerated environment. ...
- Anaerobic Bacteria. Anaerobic bacteria are used in wastewater treatment on a normal basis. ...
- Facultative.
What are the types of microorganisms used in wastewater treatment?
Common Microorganisms Used in Wastewater Treatment 1 Aerobic Bacteria. Aerobic bacteria are mostly used in new treatment plants in what is known as an aerated environment. 2 Anaerobic Bacteria. Anaerobic bacteria are used in wastewater treatment on a normal basis. ... 3 Facultative. ...
Which organisms will dominate in the future of wastewater treatment?
The organisms that will dominate will be the ones that are best suited to the environment.1 Microorganisms that are natural to the wastewater environment play a vital role in the wastewater treatment process. Beneficial bacteria, protozoa, metazoa, algae, and fungi feed on organic material in wastewater, breaking it down.
What are wastewater bacteria and what do they do?
Beneficial bacteria, protozoa, metazoa, algae, and fungi feed on organic material in wastewater, breaking it down. Bacteria clump together, or floc, forming masses that settle and separate from wastewater liquids. This settled mass is called sludge. This week, we’re going to talk specifically about wastewater bacteria.
What is biological wastewater treatment?
What are they used for? Biological wastewater treatment is the most common method of sanitation in the world. This technology uses different types of bacteria and other micro-organisms for the treatment and cleaning of polluted water. Wastewater treatment is as essential to human health as it is to environmental protection.

Which organism is commonly found in wastewater?
Bacteria. Shigella, Campylobacter, and Salmonella are the most common bacterial causes of gastroenteritis in Australia and industrialized countries but these organisms are present in low numbers in wastewater (Matthews et al., 2010).
What is organisms in wastewater?
Common Metazoa in Wastewater These organisms feed on microbial floc and protozoa. An abundance of metazoa indicates an older sludge. Common wastewater metazoa include rotifers, nematodes, bristle worms and tardigrades.
Why are microorganisms used at a wastewater treatment plant?
Microorganisms are the workhorses of wastewater treatment systems and anaerobic digesters, where they are responsible for removal of pollutants and pathogens, recovery of nutrients and energy, and producing clean water.
What microorganisms are used in secondary wastewater treatment?
Microorganisms used in a secondary treatment system include: bacteria, fungi, algae, and protozoa. These play an essential role in natural systems, and are harnessed in technologies such as activated sludge and fixed biofilm technologies such as roughing filters and slow sand filters.
What types of microorganisms are in the activated sludge?
Activated sludge (AS) is composed of aerobic and anaerobic microorganisms such as bacteria, archaea, fungi, and protists. It is capable of degrading organic compounds, including petroleum products, toluene, and benzopyrene (Seviour and Nielsen, 2010).
How micro organisms are used in sewage management?
These anaerobic bacteria digest the bacteria and fungi in sludge. Thus, use of microbes in sewage treatment helps to remove organic matter from waste water before it is discharged in water bodies like rivers.
What is microbiology of wastewater treatment?
A wastewater treatment plant is a microbiological zoo that houses bacteria, protozoa, metazoa and other microlife. The microorganisms do the actual breakdown and removal of nutrients and organic material in the wastewater. Like you and I, they perform best when all their needs are met (food, pleasant environment etc.).
What are the common protozoa in wastewater?
Common Protozoa in Wastewater. In wastewater treatment systems where there is a low load and high sludge retention time the presence of protozoa such a ciliates, flagellates , and amoebae is very common. These eukaryotic organisms are able to feed on particulates, such as suspended bacteria. It is generally assumed that protozoa's primary role in ...
What is the role of protozoa in wastewater treatment?
It is generally assumed that protozoa's primary role in wastewater treatment is the clarification of the effluent. The US Environmental Protection Agency defines effluent as discharge of liquid waste from a wastewater treatment facility, factory, or industry to a local water body.
What is metazoa in wastewater?
As a wastewater treatment system matures, dissolved oxygen concentrations eventually rise to levels that can support multicellular lifeforms. Unlike bacteria (prokaryotes) and protozoa (eukaryotes) which are all single cellular organisms, metazoa are more complex organisms with differentiated cells.
What is a wastewater treatment microscope?
A wastewater treatment microscope can be used to identify a number of different organisms during the water treatment process. Below is a guide to identifying these organisms and a few notes on what the organism identification might indicate.
What are the most common forms of stalks?
It is important to note any change in their frequency taking note of the following: Single stalk forms - most common. Colonial stalks - if seen, you have great quality water. Suctorians - similar to a stalk, but these look like a pin cushion and will suck cytoplasm from passing flagellates and ciliates.
What are nematodes in a floc?
Nematodes aid the floc with oxygen utilization. They are commonly found in healthy attached growth systems and are indicative of poor operating conditions if they are found in activated sludge systems.
What is an amoeba?
Amoeba (protozoa) Amoeba are slow moving single celled protozoa. With streaming cytoplasm, amoebae envelop free bacteria, organic particles, and other food sources. In wastewater, you can often find free amoeba which are just the streaming cytoplasm inside a cell envelope.
What is biological wastewater treatment?
Biological wastewater treatment is the most common sanitation method in the world. This technology uses different types of bacteria and other microorganisms for the treatment and purification of polluted water. Wastewater treatment is as essential to human health as it is to the protection of the environment.
Why is wastewater treatment important?
Wastewater treatment is as essential to human health as it is to the protection of the environment. The use of these bacteria accelerates the process of treating pollution on a small surface: the wastewater treatment plant.
How long does it take for bacteria to colonize the environment?
The colonization of an environment by the needed bacteria and microorganisms necessary for the purification generally lasts between 4 and 8 weeks. Once again, it is the temperature that has the most influence on this growth time.
What is lipophilic bacteria?
Lipophilic bacteria are specialized in the decomposition of animal and vegetable fats and oils in urban WWTPs and industrial treatment plants. These bacteria are easily adaptable to all current treatment systems.
How to solve the presence of undesirable bacteria?
First, the solution consists of extracting as much sludge as possible and increasing aeration. The good bacteria can take several days to recover the environment.
What are the most common microorganisms?
Among fungi, Ascomycetes are the most common, accounting for 6.3 to 7.4% of micro-organisms. Then come the archaeobacteria, with Euryarcheota (1.5% of micro-organisms, Wang et al., 2014b).
What is the structure of treated wastewater called?
Usually, these organisms swarm and aggregate into a flake-like structure within the free culture called the Floc. These flocs, visible to the naked eye, contain living and dead cells of bacteria, fungi, protozoa and metabolic products.
What are the microorganisms that are natural to the wastewater environment?
Microorganisms that are natural to the wastewater environment play a vital role in the wastewater treatment process. Beneficial bacteria, protozoa, metazoa, algae, ...
How do bacteria break down organic material in wastewater?
Bacteria break down organic material in wastewater and form the floc that settles and separates solids from liquids. Controlling F/M ratio–a vital component to creating a favorable environment for wastewater bacteria–can be accomplished through the use of bioremediation products.
What are the phases of bacteria?
Five Phases of Bacteria Growth 1 During the lag phase, bacteria adapt to the environment, developing the required enzymes to digest nutrients. Bacteria break down nutrients with enzymes that only work in favorable conditions. Bacteria cannot survive if enzymes are not working properly. 2 Influent wastewater contains high levels of nutrients that bacteria use for growth and energy. Bacteria begin to grow and reproduce during the accelerated growth phase. Growing bacteria move about in search of nutrients, quickly multiplying. They do not settle to form floc. 3 During the declining growth phase, nutrient levels begin to drop and bacteria compete for nutrients. When food levels are low, bacteria slow down to conserve energy and do not grow or reproduce. Nutrients are used for energy and cell maintenance. 4 Bacteria levels remain constant during the stationary phase. They form a thick slime layer of waste products on the outside of the cell wall. This slime layer causes bacteria to clump together to form floc. 5 The number of bacteria reduces during the death phase. 1
How do aerobic bacteria use oxygen?
Aerobic bacteria use oxygen, which is added mechanically, to break down wastewater contaminants, converting it into energy. Bacteria use this energy to grow and reproduce. Anaerobic bacteria obtain oxygen from their food source. As anaerobic bacteria break down sludge, they produce methane gas.
How many phases of growth are there in aeration?
Five Phases of Bacteria Growth. The mixture of microorganisms and wastewater in the aeration tank is called “mixed liquor.”. Bacteria goes through 5 phases of growth in the mixed liquor. During the lag phase, bacteria adapt to the environment, developing the required enzymes to digest nutrients. Bacteria break down nutrients with enzymes ...
What is the name of the mass of bacteria that settles in wastewater?
Bacteria clump together, or floc, forming masses that settle and separate from wastewater liquids. This settled mass is called sludge. This week, we’re going to talk specifically about wastewater bacteria.
What is the gas produced by anaerobic bacteria?
As anaerobic bacteria break down sludge, they produce methane gas. This methane gas can be used as an alternative energy source, called biogas, to fuel the wastewater treatment process. Anaerobic bacteria can also be used to lower the amount of phosphorus in the effluent.
What are the most common microorganisms in sludge?
The most predominant microorganisms are aerobic bacteria, but there are also substantial populations of fungi and protozoa. Rotifers and nematodes are most frequently found in systems with long aeration periods. Seman (n.d.) reports (p. 36) that protozoa "Make up about 3 percent of activated sludge microorganisms".
What is the source of food for microorganisms?
One source of food for microorganisms is human wastewater, which contains, inter alia, excrement and, in some cases, ground-up food scraps. There are many types of wastewater-treatment systems, and I'll only discuss a few common ones here.
What is a biofilm?
Biofilms of bacteria, protozoa and fungi form on the media’s surfaces and eat or otherwise reduce the organic content. The filter removes a small percentage of the suspended organic matter, while the majority of the organic matter supports microorganism reproduction and cell growth from the biological oxidation and nitrification taking place in the filter. With this aerobic oxidation and nitrification, the organic solids are converted into biofilm grazed by insect larvae, snails, and worms which help maintain an optimal thickness.
What is activated sludge made of?
Wikipedia ("Activated sludge") says that the biological floc in activated-sludge systems "is largely composed of saprotrophic bacteria but also has an important protozoan flora component mainly composed of amoebae , Spirotrichs, Peritrichs including Vorticellids and a range of other filter-feeding species.
How many micrometers are a rotifer?
To see this, consider that bacteria are typically 0.5 to 5 micrometers, while rotifers are typically 0.1 to 0.5 millimeters. Even if we conservatively assume a bacterium is 5 micrometers and a rotifer is 0.1 millimeter = 100 micrometers, that implies a difference in volume and mass of roughly (100/5) 3 = ~10 4.
What is a vermifilter?
Wikipedia ("Vermifilter") reports: "Vermifilters are most commonly used for sewage treatment (either at a centralized level or in an on-site sewage treatment facility) and for agro-industrial wastewater treatment.".
What type of system is used in rural areas?
Septic system. Many residents of rural areas use onsite sewage facilities, most commonly septic systems. For more on this type of wastewater treatment, see " Microorganisms Created by Septic Systems ".
How does biological wastewater treatment work?
Biological wastewater treatment is designed to degrade pollutants dissolved in effluents by the action of microorganisms. The microorganisms utilize these substances to live and reproduce. Pollutants are used as nutrients. A prerequisite for such degradation activity, however, is that the pollutants are soluble in water and nontoxic. Degradation process can take place either in the presence of oxygen (aerobic treatment) or in the absence of oxygen (anaerobic treatment). Both these naturally occurring principles of effluent treatment give rise to fundamental differences in the technical and economic processes involved (Table 2 ).
Which algae are good for decolorizing wastewater?
Algae such as Chlorella, Oscillateria [48], and Spirogyra
What is phytoremediation in wastewater treatment?
Phytoremediation method: This is another biological method for wastewater treatment. The combination of two Latin words―plant and remedy―gave rise to the term phytoremediation. The plant, plant origin microbes, or associated microbiota are used to take up the contamination from soil or water.
Why do aerobic microorganisms need oxygen?
Aerobic microorganisms require oxygen to support their metabolic activity. In effluent treatment, oxygen is supplied to the effluent in the form of air by special aeration equipment. Bacteria use dissolved oxygen to convert organic components into carbon dioxide and biomass.
How is phytoremediation achieved?
The remediation is achieved either by retaining, elimination, or degradation in a natural way as it happens in an ecosystem. Phytoremediation is a cheaper, eco-friendly, and feasibly sustainable method for removal of dye pollutants. Moreover, the process requires little nutrient cost and also has aesthetic demand.
How is oxygen supplied to wastewater?
In conventional aerobic biological wastewater treatment processes, oxygen is usually supplied as atmospheric air, either via immersed air-bubble diffusers or surface aeration. Diffused air bubbles (via fine-bubble aeration) are added to the bulk liquid (as in an ASP, biological aerated filters (BAFs), fluidised bioreactors, etc.), or oxygen transfer occurs from the surrounding air to the bulk liquid via a liquid/air interface (as for a TF or rotating biological contactor (RBC)).
How does biogas replace fossil fuels?
In its function as a regenerative energy carrier, biogas replaces fossil fuels in the generation of process steam, heat, and electricity. The composition and quality of biogas depend on both effluent properties and process conditions such as temperature, retention time, and volume load.
Why Treat Wastewater?
It's a matter of caring for our environment and for our own health. There are a lot of good reasons why keeping our water clean is an important priority:
Wastewater treatment
The major aim of wastewater treatment is to remove as much of the suspended solids as possible before the remaining water, called effluent, is discharged back to the environment. As solid material decays, it uses up oxygen, which is needed by the plants and animals living in the water.

Common Protozoa in Wastewater
Common Bacteria in Wastewater
- All types of bacteria enter the wastewater treatment system. It is the operator's job to create an environment that will favor the floc-forming bacteria that are rod shaped.
Other Organisms in Wastewater
- Fungi / Yeast
Fungi or yeast can indicate a low pH, fermentative conditions, or phosphorous deficiency. Sometimes if present with high numbers of Thiothrix, it can indicate septic conditions in previous clarifiers or process units that feed into the aeration section of the wastewater treatment plant. … - Tetrads
Size: 1-10µm Microscope magnification required to view: 400x, 1000x preferable A tetrad appears as a square of four cocci (arrows). It is difficult with a conventional light microscope to tell a tetrad arrangement (square of four cocci) from a sarcina arrangement (cube of eight). In waste…
Microscope Questions?
- View the wastewater treatment microscopes or contact Microscope Worldwith questions regarding which wastewater treatment microscope is best for your application. 1. Wisconsin Wastewater Operator's Association 2. Biological Wastewater Expert 3. A Guide to Higher Lifeforms
Aerobic, Anaerobic and Facultative Bacteria
Food-To-Microorganism Ratio
- The amount of food available to microorganisms is calculated by the food-to-microorganism (F/M) ratio. The F/M ratio is the measurement of incoming food divided by the microorganisms (in lbs.) in the system. This is determined by dividing results of the BOD/COD testby the mixed liquor volatile suspended solids (MLVSS). If the mixed liquor is not held long enough for nutrient level…
Bioremediation
- The F/M ratio can be adjusted with bioremediation, which is a process of treating wastewater to encourage the growth of existing microorganisms. With biostimulation, supplemental nutrients, vitamins, minerals, organic acids, and pH buffers are introduced into wastewater to create a hospitable environment that stimulates naturally occurring microorganisms. Bioaugmentation, t…
Conclusion
- Bacteria break down organic material in wastewater and form the floc that settles and separates solids from liquids. Controlling F/M ratio–a vital component to creating a favorable environment for wastewater bacteria–can be accomplished through the use of bioremediation products. The presence and activities of bacteria and other microorganisms indicates the condition of wastew…
Citations
- T Glymph (2005). Wastewater Microbiology: A Handbook for Operators, https://www.iowaruralwater.org/tools_tips/toni_glymp/Bacteria-Protozoa.pdf
- Kentucky Department for Environmental Protection (2012). Types of Bacteria Used in Wastewater Treatment, https://kyocp.wordpress.com/2012/06/14/types-of-bacteria-used-in-wastewater-treatment/
- T Glymph (2005). Wastewater Microbiology: A Handbook for Operators, https://www.iowaruralwater.org/tools_tips/toni_glymp/Bacteria-Protozoa.pdf
- Kentucky Department for Environmental Protection (2012). Types of Bacteria Used in Wastewater Treatment, https://kyocp.wordpress.com/2012/06/14/types-of-bacteria-used-in-wastewater-treatment/
- R Fuller (2017). Food-to-Mass (F:M) Ratio, The Wastewater Blog, https://www.thewastewaterblog.com/single-post/2016/12/19/Food-to-Mass-Ratio
- LM Coelho, HC Rezende, LM Coelho, PAR de Sousa, DFO Melo, and NMM Coelho (2015). Bioremediation of Polluted Waters Using Microorganisms, https://www.intechopen.com/books/advances-in-bioremediation-...