What is a replicate in research?
Replicates are multiple experimental runs with the same factor settings (levels). Replicates are subject to the same sources of variability, independently of each other. You can replicate combinations of factor levels, groups of factor level combinations, or entire designs.
What are the different types of replicates in analytical methods?
There are different types of replicates in analytical methods, which are used for different purposes. Most commonly it comes down to two types, either preparation replicates or measurement replicates.
What is a replicate in calibration?
Replicates in calibration standard preparation are often used to provide confidence in the initial weighing of the standard material. The idea is that if the response is corrected using the actual amount which was weighed out, then the response factors will be the same if the weighing has been performed correctly.
What are the advantages of using multiple replicates?
If you are trying to create a prediction model, multiple replicates can increase the precision of your model. If you have more data, you might be able to detect smaller effects or have greater power to detect an effect of fixed size. Your resources can dictate the number of replicates you can run.
How do you replicate a research treatment?
1:145:41How To Think About Replication (LE: Module 2, Part 6) - YouTubeYouTubeStart of suggested clipEnd of suggested clipIf there are very slight genetic differences from sample to sample that effect its response to aMoreIf there are very slight genetic differences from sample to sample that effect its response to a particular treatment by increasing the number of biological.
What is a replicate in an experiment?
In statistics, replication is repetition of an experiment or observation in the same or similar conditions. Replication is important because it adds information about the reliability of the conclusions or estimates to be drawn from the data. The statistical methods that assess that reliability rely on replication.
What is a replicate example?
An example of something replicate is a leaf folded in half. The definition of a replicate is a repeat of something. An example of a replicate is an experiment in cell generation which is repeated. (biology) To reproduce or make an exact copy or copies of (genetic material, a cell, or an organism).
What is the purpose of a replicate?
Replicates can be used to measure variation in the experiment so that statistical tests can be applied to evaluate differences. Averaging across replicates increases the precision of gene expression measurements and allows smaller changes to be detected.
What replicate means?
to repeat or duplicate: to repeat or duplicate (as an experiment) intransitive verb. : to undergo replication : produce a replica of itself virus particles replicating in cells. replicate.
What is a replicate in chemistry?
Getting the same result when an experiment is repeated is called replication. If research results can be replicated, it means they are more likely to be correct. Repeated replication of investigations may turn a hypothesis into a theory.
How do you use replicate?
Replicate sentence example. You might need to replicate everything. Maybe it was a primal need to replicate. The proposed model may replicate the weaknesses of the supervision model already in place.
What does replicate mean in psychology?
Thomas Barwick / Stone / Getty Images. Replication is a term referring to the repetition of a research study, generally with different situations and different subjects, to determine if the basic findings of the original study can be applied to other participants and circumstances.
What are the three steps of replication?
Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment.
What is the difference between replicate and repeat?
Repeat and replicate measurements are both multiple response measurements taken at the same combination of factor settings; but repeat measurements are taken during the same experimental run or consecutive runs, while replicate measurements are taken during identical but different experimental runs, which are often ...
What is a true replicate?
True replication permits the estimation of variability within a treatment. Without estimating variability within treatments, it is impossible to do statistical inference. Consider, for example, comparing two drugs by trying drug A on person 1 and drug B on person 2.
Example
As an example, consider a continuous process which produces items. Batches of items are then processed or treated. Finally, tests or measurements are conducted. Several options might be available to obtain ten test values. Some possibilities are:
Bibliography
ASTM E122-07 Standard Practice for Calculating Sample Size to Estimate, With Specified Precision, the Average for a Characteristic of a Lot or Process
Popular Answers (1)
Experimental unit: This is the (field plot/animal/gear/whatever) to which "treatments" are applied. Treatments can be directly applied (like a dose of insecticide to an insect) or they can be observational (sex, weather, disease). If you randomize, you typically randomize the experimental units.
All Answers (9)
Experimental unit: This is the (field plot/animal/gear/whatever) to which "treatments" are applied. Treatments can be directly applied (like a dose of insecticide to an insect) or they can be observational (sex, weather, disease). If you randomize, you typically randomize the experimental units.
Similar questions and discussions
10 samples x 3 replicates, 5 samples x 6 replicates or 1 samples x 30 replicates, is there any difference?
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What is replication in science?
What is replication in an experiment? In engineering, science, and statistics, replication is the repetition of an experimental condition so that the variability associated with the phenomenon can be estimated.
Why is replication important in science?
Replication is important in science so scientists can “check their work.”. The result of an investigation is not likely to be well accepted unless the investigation is repeated many times ...
When does replication occur?
Replication occurs when a scientific investigation is reproduced by another person. Repetition is multiple trials, when replication is when you redo the entire experiment.
What is the act of reproducing or copying something?
Replication is the act of reproducing or copying something, or is a copy of something. When an experiment is repeated and the results from the original are reproduced, this is an example of a replication of the original study. A copy of a Monet painting is an example of areplication .
Why is replication used in sample preparation?
When replicates are used in sample preparation it is more likely that the reason is related to variability of the method caused by the inherent errors in the sample preparation steps. When the sample is very simple, such as a pure substance then the replication may be used a weighing check as discussed for standards previously.
What are the two types of replicates?
Most commonly it comes down to two types, either preparation replicates or measurement replicates. In the case of preparation, these are samples or standards that are prepared from the beginning to end of the procedure in ...
What is replicate in a study?
Replicates are multiple experimental runs with the same factor settings (levels). Replicates are subject to the same sources of variability, independently of each other. You can replicate combinations of factor levels, groups of factor level combinations, or entire designs. For example, if you have three factors with two levels each ...
What is the difference between repeat and replicate?
Repeat and replicate measurements are both multiple response measurements taken at the same combination of factor settings; but repeat measurements are taken during the same experimental run or consecutive runs, while replicate measurements are taken during identical but different experimental runs, which are often randomized.
Why is variability greater for replicates than for repeats?
The variability between measurements taken at the same factor settings tends to be greater for replicates than for repeats because the machines are reset before each run, adding more variability to the process.
How does DNA replication work?
And we start out from a single cell and we end up with trillions of cells. And during that process of cell division, all of the information in a cell has to be copied, and it has to be copied perfectly. And so DNA is a molecule that can be replicated to make almost perfect copies of itself. Which is all the more amazing considering that there are almost three billion base pairs of DNA to be copied. And replication uses DNA polymerases which are molecules specifically dedicated to just copying DNA. Replicating all of the DNA in a single human cell takes several hours of just pure copying time. At the end of this process, once the DNA is all replicated, the cell actually has twice the amount of DNA that it needs, and the cell can then divide and parcel this DNA into the daughter cell, so that the daughter cell and the parental cell in many case are absolutely genetically identical.
What is the process by which a molecule of DNA is duplicated?
DNA Replication . DNA replication is the process by which a molecule of DNA is duplicated.
What happens to DNA at the end of the process?
At the end of this process, once the DNA is all replicated, the cell actually has twice the amount of DNA that it needs, and the cell can then divide and parcel this DNA into the daughter cell, so that the daughter cell and the parental cell in many case are absolutely genetically identical. Lawrence C. Brody, Ph.D.
Can DNA be replicated?
And so DNA is a molecule that can be replicated to make almost perfect copies of itself. Which is all the more amazing considering that there are almost three billion base pairs of DNA to be copied. And replication uses DNA polymerases which are molecules specifically dedicated to just copying DNA.
Why do you need replications in statistical anaylsis?
You need replications to see how the measures change in different situations and understand the behavior of your observations.
What is repetition in science?
Repetition is when you take different measurements during the same experiment. For example, if I have three temperatures and i wanna know their effect on seaweed growth, in each treatment I going to have 4 repetitions, that means, 4 culture vessels in which the seaweed grows.
