If you are doing experiment treating cells with some chemical, you dissovle chemical A in B to make a solution for treatment, you will have to add the same amount of B in Mock treatment which sometimes is also called vechicle treatment. Hope I have made it clear Mairo
What is a mock transfection?
what is Mock? is that DNA? and why do we use it in mammalian transfection as a negative control? i have checked the net, but can't find any info about it. Mock transfection is transfection without DNA to control potential effect of transfection reagent on the cells.
What is mock treatment in chemistry?
If you are doing experiment treating cells with some chemical, you dissovle chemical A in B to make a solution for treatment, you will have to add the same amount of B in Mock treatment which sometimes is also called vechicle treatment. thanks.
What can be used as mock?
thanks, so what can be used as Mock? For example, you are transfecting an expressing vector with lipofectamine, the mock will be lipofectamine only with DNA replaced by medium.
What is a mock-infected specimen?
Two specimens are used one that is infected with the virus / vector of interest and the other is treated the same way except without the virus. Sometimes a non-virulent strain is used in the mock-infected specimen. If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.
What is mock treatment?
In pre-clinical biomedical research, people often refer to control samples as "mock-treated", e.g. cell lines treated with culture medium or a solvent, without an active substance (drug). It can be regarded as an equivalent of a placebo-treated group in a clinical trial.
What does mock mean in science?
Imitating reality, but not real; false; counterfeit; assumed; sham. ”That superior greatness and mock majesty. (Science: botany) ” (Spectator) Mock bishop's weed, a genus of American and Asiatic shrubs (Philadelphus), with showy white flowers in panicled cymes.
What is a mock experiment?
The Mock Experiment can be used to introduce science and nonscience majors to the scientific process, presentation of research, and literature. The Mock Experiment leads students by application through literature review, experiment development, manuscript preparation, and scientific presentation.
What is a mock cell line?
a mock setting is a control setting where you do exactly the same as in your experimental set-up except you leave out the specific stimulus you want to provide. Maybe it's more comprehensible with an example: Let's say you want to study the effects of p53 overexpression in a cell line.
What is mock in molecular biology?
A control used in infection experiments. Two specimens are used one that is infected with the virus/vector of interest and the other is treated the same way except without the virus. Sometimes a non-virulent strain is used in the mock-infected specimen.
What do you mean by mock?
noun. Definition of mock (Entry 2 of 4) 1 : an act of ridicule or derision : jeer. 2 : one that is an object of derision or scorn. 3 : mockery.
What is mock sample?
2 : a working sample (as of a magazine) for reviewing format, layout, or content.
What is the part of the experiment that does not change?
Control Variable: Any variable that does not change during an experiment. Also known as a constant variable.
What is a negative control for an experiment?
Negative controls are particular samples included in the experiment that are treated the same as all the others but are not expected to change from any variable in the experiment.
What is the purpose of a mock infection?
mock-infected definition. A control used in infection experiments. Two specimens are used one that is infected with the virus/vector of interest and the other is treated the same way except without the virus. Sometimes a non-virulent strain is used in the mock-infected specimen.
What is a mock transfection?
Mock Transfection Treatment of cells with only the delivery agent (e.g. transfection reagent alone or empty vector) to control for cellular effects due to transfection reagent exposure or transduction. This control is important because transfection reagents or transduction can easily alter gene expression.
What is transfection method?
Transfection is a procedure that introduces foreign nucleic acids into cells to produce genetically modified cells. Transfection is a powerful analytical tool for study of gene function and regulation and protein function.