BT-474 will take approx.30-50 days for palpable tumors to appear,or else you can try implanting tumor fragments for the same. T47D takes 45-60 days for tumors to appear.These figures are from my experience.might vary with lab to lab depend on the cells and conditions, Better use SCID mice for the above.Good luck!
Full Answer
What can we learn from the BT474 mouse xenograft model of breast cancer?
Preclinical studies of the BT474 mouse xenograft model can be used to evaluate anti-cancer therapies. Well-studied breast tumor cell lines are indispensable tools for investigating cell biology of carcinomas. Breast cancer cell lines are extensively employed in preclinical research as molecular models of different types of breast tumors.
What is the BT474 cell line used for?
The BT474 cell line is used to create the CDX (Cell Line Derived Xenograft) BT474 xenograft mouse model. The BT474 breast cancer model can be used to screen anti-cancer therapeutics related to the standard of care drugs (herceptin, lapatinib, trastuzumab) or hormone-induced disease progression (progesterone, norgestrel, norethindrone and MPA).
What is the role of xenograft in breast cancer research?
Breast cancer in humans is extremely heterogeneous, thus, any therapeutic studies performed in vitro must be followed by a xenograft study.
Can I use alternative products for BT-474?
If you use alternative products, the quality and effectiveness of the item may be affected. The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range.
What happens when you thaw a liquid nitrogen vial?
Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Is XO aneuploid or hypertetraploid?
The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der (14)t (14;?) (q32,?), unknown, iso (13q), der (6)t (6;7) (q21;q21), der (11)t (11;?) (14;?), del (11) (p11), unknown, unknown, der (2)t (2;?) (p21;?). Several of the latter were reported by E. Lasfargues, et al.
Does ATCC warrant citations?
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